Real-space visualization of a single icosahedral protein cage via IR PiFM

Characterization of an icosahedral protein cage with Fourier-filtered scanning EM, AFM topography and PiFM.

Characterization of an icosahedral protein cage by bright field Fourier-filtered scanning EM, AFM topography, and PiFM, along with a model view. The EM image reveals the hexagonal feature. The PiFM images (based on Amide I signal at 1666 cm-1) clearly reveal not only the hexagonal-like outline but also the facets of the cage.

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