E-coli Bacteria on Mica Substrate

E-coli bacteria on mica substrate imaged with PiFM

E-coli bacteria is imaged via amide I band (1660 cm-1). While the topography cannot show the fimbriae due to the extreme height difference, PiFM image highlights all the parts of bacteria that are exhibiting amide I band, including fimbriae. The lower right image overlays the PiFM map of mica (acquired at 1030 cm-1) and the distribution of amide I band (acquired at 1660 cm-1) onto the 3D topography. Note that the amide I image shows details that are hard to glean from the topography.

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